puc‐t vector (Beijing CWBio)
90
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Beijing CWBio
puc‐t vector
Puc‐T Vector, supplied by Beijing CWBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/puc-t+vector/puc+t+vector/pmc11740985-76-14-18
Average 90 stars, based on 1 article reviews
Puc‐T Vector, supplied by Beijing CWBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/puc-t+vector/puc+t+vector/pmc11740985-76-14-18
Average 90 stars, based on 1 article reviews
puc‐t vector - by Bioz Stars,
2026-09
90/100 stars
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Polymerase Chain Reaction:Article Title: miR-29c-3p regulates DNMT3B and LATS1 methylation to inhibit tumor progression in hepatocellular carcinoma Article Snippet: The PCR products were electrophoresed, purified using Spin‐X tubes, and then cloned into the pUC-T vector (both from CWbiotech, Beijing, China). Article Title: Modified plants Article Snippet: The PCR products amplified with TaMLO-specific primers (Table 3) from individual mutant plants were cloned into Article Title: Methylation-associated inactivation of JPH3 and its effect on prognosis and cell biological function in HCC Article Snippet: The PCR products were electrophoresed, purified using Spin-X tubes, and then cloned into the Article Title: Advances in Cereal Crop Genomics for Resilience under Climate Change Article Snippet: , AMT , MLO , NHEJ , TaU6 , Ubi , Article Title: Complete nucleotide sequence of strawberry vein banding virus Chinese isolate and infectivity of its full-length DNA clone Article Snippet: The PCR products with a 3′-A addition were inserted into pUC-T Vector (CWBIO, Beijing, China) using TA-cloning strategy followed by transformation of chemically competent Escherichia coli DH5α cells [ ]. Article Title: Molecular cloning, the characterization of metallothionein and catalase, and the evaluation of testicular toxicity of Cd in the Chinese fire-bellied newt (Cynops orientalis). Article Snippet: All products that were produced by the PCRs were isolated via electrophoresis, purified using Article Title: Genome editing in rice and wheat using the CRISPR/Cas system. Article Snippet: nature protocols | VOL.9 NO.10 | 2014 | 2395 IntroDuctIon In recent years, genome editing technologies, ZFNs1–3 and TALENs4–8 have been used to mutate specific loci in various plants9–16.. Both ZFNs and TALENs are fusion proteins that consist of a DNA-binding domain fused to the FokI endonuclease domain.. The endonuclease domain induces DNA double-strand breaks (DSBs) at targeted sites in the genome, which are determined by the binding specificity of the DNA-binding domain. Article Title: Wheat plants resistant to powdery mildew Article Snippet: The PCR products amplified with TaMLO-specific primers (Table 3) from individual mutant plants were cloned into Purification:Article Title: miR-29c-3p regulates DNMT3B and LATS1 methylation to inhibit tumor progression in hepatocellular carcinoma Article Snippet: The PCR products were electrophoresed, purified using Spin‐X tubes, and then cloned into the pUC-T vector (both from CWbiotech, Beijing, China). Article Title: Modified plants Article Snippet: The PCR products amplified with TaMLO-specific primers (Table 3) from individual mutant plants were cloned into Article Title: Methylation-associated inactivation of JPH3 and its effect on prognosis and cell biological function in HCC Article Snippet: The PCR products were electrophoresed, purified using Spin-X tubes, and then cloned into the Article Title: Advances in Cereal Crop Genomics for Resilience under Climate Change Article Snippet: , AMT , MLO , NHEJ , TaU6 , Ubi , Article Title: Complete nucleotide sequence of strawberry vein banding virus Chinese isolate and infectivity of its full-length DNA clone Article Snippet: The PCR products with a 3′-A addition were inserted into pUC-T Vector (CWBIO, Beijing, China) using TA-cloning strategy followed by transformation of chemically competent Escherichia coli DH5α cells [ ]. Article Title: Molecular cloning, the characterization of metallothionein and catalase, and the evaluation of testicular toxicity of Cd in the Chinese fire-bellied newt (Cynops orientalis). Article Snippet: All products that were produced by the PCRs were isolated via electrophoresis, purified using Article Title: Genome editing in rice and wheat using the CRISPR/Cas system. Article Snippet: nature protocols | VOL.9 NO.10 | 2014 | 2395 IntroDuctIon In recent years, genome editing technologies, ZFNs1–3 and TALENs4–8 have been used to mutate specific loci in various plants9–16.. Both ZFNs and TALENs are fusion proteins that consist of a DNA-binding domain fused to the FokI endonuclease domain.. The endonuclease domain induces DNA double-strand breaks (DSBs) at targeted sites in the genome, which are determined by the binding specificity of the DNA-binding domain. Article Title: Wheat plants resistant to powdery mildew Article Snippet: The PCR products amplified with TaMLO-specific primers (Table 3) from individual mutant plants were cloned into Clone Assay:Article Title: miR-29c-3p regulates DNMT3B and LATS1 methylation to inhibit tumor progression in hepatocellular carcinoma Article Snippet: The PCR products were electrophoresed, purified using Spin‐X tubes, and then cloned into the pUC-T vector (both from CWbiotech, Beijing, China). Article Title: Modified plants Article Snippet: The PCR products amplified with TaMLO-specific primers (Table 3) from individual mutant plants were cloned into Article Title: Methylation-associated inactivation of JPH3 and its effect on prognosis and cell biological function in HCC Article Snippet: The PCR products were electrophoresed, purified using Spin-X tubes, and then cloned into the Article Title: Advances in Cereal Crop Genomics for Resilience under Climate Change Article Snippet: , AMT , MLO , NHEJ , TaU6 , Ubi , Article Title: Complete nucleotide sequence of strawberry vein banding virus Chinese isolate and infectivity of its full-length DNA clone Article Snippet: The PCR products with a 3′-A addition were inserted into pUC-T Vector (CWBIO, Beijing, China) using TA-cloning strategy followed by transformation of chemically competent Escherichia coli DH5α cells [ ]. Article Title: Molecular cloning, the characterization of metallothionein and catalase, and the evaluation of testicular toxicity of Cd in the Chinese fire-bellied newt (Cynops orientalis). Article Snippet: All products that were produced by the PCRs were isolated via electrophoresis, purified using Article Title: Genome editing in rice and wheat using the CRISPR/Cas system. Article Snippet: nature protocols | VOL.9 NO.10 | 2014 | 2395 IntroDuctIon In recent years, genome editing technologies, ZFNs1–3 and TALENs4–8 have been used to mutate specific loci in various plants9–16.. Both ZFNs and TALENs are fusion proteins that consist of a DNA-binding domain fused to the FokI endonuclease domain.. The endonuclease domain induces DNA double-strand breaks (DSBs) at targeted sites in the genome, which are determined by the binding specificity of the DNA-binding domain. Article Title: Wheat plants resistant to powdery mildew Article Snippet: The PCR products amplified with TaMLO-specific primers (Table 3) from individual mutant plants were cloned into Plasmid Preparation:Article Title: miR-29c-3p regulates DNMT3B and LATS1 methylation to inhibit tumor progression in hepatocellular carcinoma Article Snippet: The PCR products were electrophoresed, purified using Spin‐X tubes, and then cloned into the pUC-T vector (both from CWbiotech, Beijing, China). Article Title: Modified plants Article Snippet: The PCR products amplified with TaMLO-specific primers (Table 3) from individual mutant plants were cloned into Article Title: Methylation-associated inactivation of JPH3 and its effect on prognosis and cell biological function in HCC Article Snippet: The PCR products were electrophoresed, purified using Spin-X tubes, and then cloned into the Article Title: Advances in Cereal Crop Genomics for Resilience under Climate Change Article Snippet: , AMT , MLO , NHEJ , TaU6 , Ubi , Article Title: Complete nucleotide sequence of strawberry vein banding virus Chinese isolate and infectivity of its full-length DNA clone Article Snippet: The PCR products with a 3′-A addition were inserted into pUC-T Vector (CWBIO, Beijing, China) using TA-cloning strategy followed by transformation of chemically competent Escherichia coli DH5α cells [ ]. Article Title: Molecular cloning, the characterization of metallothionein and catalase, and the evaluation of testicular toxicity of Cd in the Chinese fire-bellied newt (Cynops orientalis). Article Snippet: All products that were produced by the PCRs were isolated via electrophoresis, purified using Article Title: Genome editing in rice and wheat using the CRISPR/Cas system. Article Snippet: nature protocols | VOL.9 NO.10 | 2014 | 2395 IntroDuctIon In recent years, genome editing technologies, ZFNs1–3 and TALENs4–8 have been used to mutate specific loci in various plants9–16.. Both ZFNs and TALENs are fusion proteins that consist of a DNA-binding domain fused to the FokI endonuclease domain.. The endonuclease domain induces DNA double-strand breaks (DSBs) at targeted sites in the genome, which are determined by the binding specificity of the DNA-binding domain. Article Title: Wheat plants resistant to powdery mildew Article Snippet: The PCR products amplified with TaMLO-specific primers (Table 3) from individual mutant plants were cloned into Non-Homologous End Joining:Article Title: miR-29c-3p regulates DNMT3B and LATS1 methylation to inhibit tumor progression in hepatocellular carcinoma Article Snippet: The PCR products were electrophoresed, purified using Spin‐X tubes, and then cloned into the pUC-T vector (both from CWbiotech, Beijing, China). Article Title: Modified plants Article Snippet: The PCR products amplified with TaMLO-specific primers (Table 3) from individual mutant plants were cloned into Article Title: Methylation-associated inactivation of JPH3 and its effect on prognosis and cell biological function in HCC Article Snippet: The PCR products were electrophoresed, purified using Spin-X tubes, and then cloned into the Article Title: Advances in Cereal Crop Genomics for Resilience under Climate Change Article Snippet: , AMT , MLO , NHEJ , TaU6 , Ubi , Article Title: Complete nucleotide sequence of strawberry vein banding virus Chinese isolate and infectivity of its full-length DNA clone Article Snippet: The PCR products with a 3′-A addition were inserted into pUC-T Vector (CWBIO, Beijing, China) using TA-cloning strategy followed by transformation of chemically competent Escherichia coli DH5α cells [ ]. Article Title: Molecular cloning, the characterization of metallothionein and catalase, and the evaluation of testicular toxicity of Cd in the Chinese fire-bellied newt (Cynops orientalis). Article Snippet: All products that were produced by the PCRs were isolated via electrophoresis, purified using Article Title: Genome editing in rice and wheat using the CRISPR/Cas system. Article Snippet: nature protocols | VOL.9 NO.10 | 2014 | 2395 IntroDuctIon In recent years, genome editing technologies, ZFNs1–3 and TALENs4–8 have been used to mutate specific loci in various plants9–16.. Both ZFNs and TALENs are fusion proteins that consist of a DNA-binding domain fused to the FokI endonuclease domain.. The endonuclease domain induces DNA double-strand breaks (DSBs) at targeted sites in the genome, which are determined by the binding specificity of the DNA-binding domain. Article Title: Wheat plants resistant to powdery mildew Article Snippet: The PCR products amplified with TaMLO-specific primers (Table 3) from individual mutant plants were cloned into CRISPR:Article Title: miR-29c-3p regulates DNMT3B and LATS1 methylation to inhibit tumor progression in hepatocellular carcinoma Article Snippet: The PCR products were electrophoresed, purified using Spin‐X tubes, and then cloned into the pUC-T vector (both from CWbiotech, Beijing, China). Article Title: Modified plants Article Snippet: The PCR products amplified with TaMLO-specific primers (Table 3) from individual mutant plants were cloned into Article Title: Methylation-associated inactivation of JPH3 and its effect on prognosis and cell biological function in HCC Article Snippet: The PCR products were electrophoresed, purified using Spin-X tubes, and then cloned into the Article Title: Advances in Cereal Crop Genomics for Resilience under Climate Change Article Snippet: , AMT , MLO , NHEJ , TaU6 , Ubi , Article Title: Complete nucleotide sequence of strawberry vein banding virus Chinese isolate and infectivity of its full-length DNA clone Article Snippet: The PCR products with a 3′-A addition were inserted into pUC-T Vector (CWBIO, Beijing, China) using TA-cloning strategy followed by transformation of chemically competent Escherichia coli DH5α cells [ ]. Article Title: Molecular cloning, the characterization of metallothionein and catalase, and the evaluation of testicular toxicity of Cd in the Chinese fire-bellied newt (Cynops orientalis). Article Snippet: All products that were produced by the PCRs were isolated via electrophoresis, purified using Article Title: Genome editing in rice and wheat using the CRISPR/Cas system. Article Snippet: nature protocols | VOL.9 NO.10 | 2014 | 2395 IntroDuctIon In recent years, genome editing technologies, ZFNs1–3 and TALENs4–8 have been used to mutate specific loci in various plants9–16.. Both ZFNs and TALENs are fusion proteins that consist of a DNA-binding domain fused to the FokI endonuclease domain.. The endonuclease domain induces DNA double-strand breaks (DSBs) at targeted sites in the genome, which are determined by the binding specificity of the DNA-binding domain. Article Title: Wheat plants resistant to powdery mildew Article Snippet: The PCR products amplified with TaMLO-specific primers (Table 3) from individual mutant plants were cloned into Sterility:Article Title: miR-29c-3p regulates DNMT3B and LATS1 methylation to inhibit tumor progression in hepatocellular carcinoma Article Snippet: The PCR products were electrophoresed, purified using Spin‐X tubes, and then cloned into the pUC-T vector (both from CWbiotech, Beijing, China). Article Title: Modified plants Article Snippet: The PCR products amplified with TaMLO-specific primers (Table 3) from individual mutant plants were cloned into Article Title: Methylation-associated inactivation of JPH3 and its effect on prognosis and cell biological function in HCC Article Snippet: The PCR products were electrophoresed, purified using Spin-X tubes, and then cloned into the Article Title: Advances in Cereal Crop Genomics for Resilience under Climate Change Article Snippet: , AMT , MLO , NHEJ , TaU6 , Ubi , Article Title: Complete nucleotide sequence of strawberry vein banding virus Chinese isolate and infectivity of its full-length DNA clone Article Snippet: The PCR products with a 3′-A addition were inserted into pUC-T Vector (CWBIO, Beijing, China) using TA-cloning strategy followed by transformation of chemically competent Escherichia coli DH5α cells [ ]. Article Title: Molecular cloning, the characterization of metallothionein and catalase, and the evaluation of testicular toxicity of Cd in the Chinese fire-bellied newt (Cynops orientalis). Article Snippet: All products that were produced by the PCRs were isolated via electrophoresis, purified using Article Title: Genome editing in rice and wheat using the CRISPR/Cas system. Article Snippet: nature protocols | VOL.9 NO.10 | 2014 | 2395 IntroDuctIon In recent years, genome editing technologies, ZFNs1–3 and TALENs4–8 have been used to mutate specific loci in various plants9–16.. Both ZFNs and TALENs are fusion proteins that consist of a DNA-binding domain fused to the FokI endonuclease domain.. The endonuclease domain induces DNA double-strand breaks (DSBs) at targeted sites in the genome, which are determined by the binding specificity of the DNA-binding domain. Article Title: Wheat plants resistant to powdery mildew Article Snippet: The PCR products amplified with TaMLO-specific primers (Table 3) from individual mutant plants were cloned into Amplification:Article Title: miR-29c-3p regulates DNMT3B and LATS1 methylation to inhibit tumor progression in hepatocellular carcinoma Article Snippet: The PCR products were electrophoresed, purified using Spin‐X tubes, and then cloned into the pUC-T vector (both from CWbiotech, Beijing, China). Article Title: Modified plants Article Snippet: The PCR products amplified with TaMLO-specific primers (Table 3) from individual mutant plants were cloned into Article Title: Methylation-associated inactivation of JPH3 and its effect on prognosis and cell biological function in HCC Article Snippet: The PCR products were electrophoresed, purified using Spin-X tubes, and then cloned into the Article Title: Advances in Cereal Crop Genomics for Resilience under Climate Change Article Snippet: , AMT , MLO , NHEJ , TaU6 , Ubi , Article Title: Complete nucleotide sequence of strawberry vein banding virus Chinese isolate and infectivity of its full-length DNA clone Article Snippet: The PCR products with a 3′-A addition were inserted into pUC-T Vector (CWBIO, Beijing, China) using TA-cloning strategy followed by transformation of chemically competent Escherichia coli DH5α cells [ ]. Article Title: Molecular cloning, the characterization of metallothionein and catalase, and the evaluation of testicular toxicity of Cd in the Chinese fire-bellied newt (Cynops orientalis). Article Snippet: All products that were produced by the PCRs were isolated via electrophoresis, purified using Article Title: Genome editing in rice and wheat using the CRISPR/Cas system. Article Snippet: nature protocols | VOL.9 NO.10 | 2014 | 2395 IntroDuctIon In recent years, genome editing technologies, ZFNs1–3 and TALENs4–8 have been used to mutate specific loci in various plants9–16.. Both ZFNs and TALENs are fusion proteins that consist of a DNA-binding domain fused to the FokI endonuclease domain.. The endonuclease domain induces DNA double-strand breaks (DSBs) at targeted sites in the genome, which are determined by the binding specificity of the DNA-binding domain. Article Title: Wheat plants resistant to powdery mildew Article Snippet: The PCR products amplified with TaMLO-specific primers (Table 3) from individual mutant plants were cloned into Mutagenesis:Article Title: miR-29c-3p regulates DNMT3B and LATS1 methylation to inhibit tumor progression in hepatocellular carcinoma Article Snippet: The PCR products were electrophoresed, purified using Spin‐X tubes, and then cloned into the pUC-T vector (both from CWbiotech, Beijing, China). Article Title: Modified plants Article Snippet: The PCR products amplified with TaMLO-specific primers (Table 3) from individual mutant plants were cloned into Article Title: Methylation-associated inactivation of JPH3 and its effect on prognosis and cell biological function in HCC Article Snippet: The PCR products were electrophoresed, purified using Spin-X tubes, and then cloned into the Article Title: Advances in Cereal Crop Genomics for Resilience under Climate Change Article Snippet: , AMT , MLO , NHEJ , TaU6 , Ubi , Article Title: Complete nucleotide sequence of strawberry vein banding virus Chinese isolate and infectivity of its full-length DNA clone Article Snippet: The PCR products with a 3′-A addition were inserted into pUC-T Vector (CWBIO, Beijing, China) using TA-cloning strategy followed by transformation of chemically competent Escherichia coli DH5α cells [ ]. Article Title: Molecular cloning, the characterization of metallothionein and catalase, and the evaluation of testicular toxicity of Cd in the Chinese fire-bellied newt (Cynops orientalis). Article Snippet: All products that were produced by the PCRs were isolated via electrophoresis, purified using Article Title: Genome editing in rice and wheat using the CRISPR/Cas system. Article Snippet: nature protocols | VOL.9 NO.10 | 2014 | 2395 IntroDuctIon In recent years, genome editing technologies, ZFNs1–3 and TALENs4–8 have been used to mutate specific loci in various plants9–16.. Both ZFNs and TALENs are fusion proteins that consist of a DNA-binding domain fused to the FokI endonuclease domain.. The endonuclease domain induces DNA double-strand breaks (DSBs) at targeted sites in the genome, which are determined by the binding specificity of the DNA-binding domain. Article Title: Wheat plants resistant to powdery mildew Article Snippet: The PCR products amplified with TaMLO-specific primers (Table 3) from individual mutant plants were cloned into Sequencing:Article Title: miR-29c-3p regulates DNMT3B and LATS1 methylation to inhibit tumor progression in hepatocellular carcinoma Article Snippet: The PCR products were electrophoresed, purified using Spin‐X tubes, and then cloned into the pUC-T vector (both from CWbiotech, Beijing, China). Article Title: Modified plants Article Snippet: The PCR products amplified with TaMLO-specific primers (Table 3) from individual mutant plants were cloned into Article Title: Methylation-associated inactivation of JPH3 and its effect on prognosis and cell biological function in HCC Article Snippet: The PCR products were electrophoresed, purified using Spin-X tubes, and then cloned into the Article Title: Advances in Cereal Crop Genomics for Resilience under Climate Change Article Snippet: , AMT , MLO , NHEJ , TaU6 , Ubi , Article Title: Complete nucleotide sequence of strawberry vein banding virus Chinese isolate and infectivity of its full-length DNA clone Article Snippet: The PCR products with a 3′-A addition were inserted into pUC-T Vector (CWBIO, Beijing, China) using TA-cloning strategy followed by transformation of chemically competent Escherichia coli DH5α cells [ ]. Article Title: Molecular cloning, the characterization of metallothionein and catalase, and the evaluation of testicular toxicity of Cd in the Chinese fire-bellied newt (Cynops orientalis). Article Snippet: All products that were produced by the PCRs were isolated via electrophoresis, purified using Article Title: Genome editing in rice and wheat using the CRISPR/Cas system. Article Snippet: nature protocols | VOL.9 NO.10 | 2014 | 2395 IntroDuctIon In recent years, genome editing technologies, ZFNs1–3 and TALENs4–8 have been used to mutate specific loci in various plants9–16.. Both ZFNs and TALENs are fusion proteins that consist of a DNA-binding domain fused to the FokI endonuclease domain.. The endonuclease domain induces DNA double-strand breaks (DSBs) at targeted sites in the genome, which are determined by the binding specificity of the DNA-binding domain. Article Title: Wheat plants resistant to powdery mildew Article Snippet: The PCR products amplified with TaMLO-specific primers (Table 3) from individual mutant plants were cloned into Produced:Article Title: miR-29c-3p regulates DNMT3B and LATS1 methylation to inhibit tumor progression in hepatocellular carcinoma Article Snippet: The PCR products were electrophoresed, purified using Spin‐X tubes, and then cloned into the pUC-T vector (both from CWbiotech, Beijing, China). Article Title: Modified plants Article Snippet: The PCR products amplified with TaMLO-specific primers (Table 3) from individual mutant plants were cloned into Article Title: Methylation-associated inactivation of JPH3 and its effect on prognosis and cell biological function in HCC Article Snippet: The PCR products were electrophoresed, purified using Spin-X tubes, and then cloned into the Article Title: Advances in Cereal Crop Genomics for Resilience under Climate Change Article Snippet: , AMT , MLO , NHEJ , TaU6 , Ubi , Article Title: Complete nucleotide sequence of strawberry vein banding virus Chinese isolate and infectivity of its full-length DNA clone Article Snippet: The PCR products with a 3′-A addition were inserted into pUC-T Vector (CWBIO, Beijing, China) using TA-cloning strategy followed by transformation of chemically competent Escherichia coli DH5α cells [ ]. Article Title: Molecular cloning, the characterization of metallothionein and catalase, and the evaluation of testicular toxicity of Cd in the Chinese fire-bellied newt (Cynops orientalis). Article Snippet: All products that were produced by the PCRs were isolated via electrophoresis, purified using Article Title: Genome editing in rice and wheat using the CRISPR/Cas system. Article Snippet: nature protocols | VOL.9 NO.10 | 2014 | 2395 IntroDuctIon In recent years, genome editing technologies, ZFNs1–3 and TALENs4–8 have been used to mutate specific loci in various plants9–16.. Both ZFNs and TALENs are fusion proteins that consist of a DNA-binding domain fused to the FokI endonuclease domain.. The endonuclease domain induces DNA double-strand breaks (DSBs) at targeted sites in the genome, which are determined by the binding specificity of the DNA-binding domain. Article Title: Wheat plants resistant to powdery mildew Article Snippet: The PCR products amplified with TaMLO-specific primers (Table 3) from individual mutant plants were cloned into Isolation:Article Title: miR-29c-3p regulates DNMT3B and LATS1 methylation to inhibit tumor progression in hepatocellular carcinoma Article Snippet: The PCR products were electrophoresed, purified using Spin‐X tubes, and then cloned into the pUC-T vector (both from CWbiotech, Beijing, China). Article Title: Modified plants Article Snippet: The PCR products amplified with TaMLO-specific primers (Table 3) from individual mutant plants were cloned into Article Title: Methylation-associated inactivation of JPH3 and its effect on prognosis and cell biological function in HCC Article Snippet: The PCR products were electrophoresed, purified using Spin-X tubes, and then cloned into the Article Title: Advances in Cereal Crop Genomics for Resilience under Climate Change Article Snippet: , AMT , MLO , NHEJ , TaU6 , Ubi , Article Title: Complete nucleotide sequence of strawberry vein banding virus Chinese isolate and infectivity of its full-length DNA clone Article Snippet: The PCR products with a 3′-A addition were inserted into pUC-T Vector (CWBIO, Beijing, China) using TA-cloning strategy followed by transformation of chemically competent Escherichia coli DH5α cells [ ]. Article Title: Molecular cloning, the characterization of metallothionein and catalase, and the evaluation of testicular toxicity of Cd in the Chinese fire-bellied newt (Cynops orientalis). Article Snippet: All products that were produced by the PCRs were isolated via electrophoresis, purified using Article Title: Genome editing in rice and wheat using the CRISPR/Cas system. Article Snippet: nature protocols | VOL.9 NO.10 | 2014 | 2395 IntroDuctIon In recent years, genome editing technologies, ZFNs1–3 and TALENs4–8 have been used to mutate specific loci in various plants9–16.. Both ZFNs and TALENs are fusion proteins that consist of a DNA-binding domain fused to the FokI endonuclease domain.. The endonuclease domain induces DNA double-strand breaks (DSBs) at targeted sites in the genome, which are determined by the binding specificity of the DNA-binding domain. Article Title: Wheat plants resistant to powdery mildew Article Snippet: The PCR products amplified with TaMLO-specific primers (Table 3) from individual mutant plants were cloned into Electrophoresis:Article Title: miR-29c-3p regulates DNMT3B and LATS1 methylation to inhibit tumor progression in hepatocellular carcinoma Article Snippet: The PCR products were electrophoresed, purified using Spin‐X tubes, and then cloned into the pUC-T vector (both from CWbiotech, Beijing, China). Article Title: Modified plants Article Snippet: The PCR products amplified with TaMLO-specific primers (Table 3) from individual mutant plants were cloned into Article Title: Methylation-associated inactivation of JPH3 and its effect on prognosis and cell biological function in HCC Article Snippet: The PCR products were electrophoresed, purified using Spin-X tubes, and then cloned into the Article Title: Advances in Cereal Crop Genomics for Resilience under Climate Change Article Snippet: , AMT , MLO , NHEJ , TaU6 , Ubi , Article Title: Complete nucleotide sequence of strawberry vein banding virus Chinese isolate and infectivity of its full-length DNA clone Article Snippet: The PCR products with a 3′-A addition were inserted into pUC-T Vector (CWBIO, Beijing, China) using TA-cloning strategy followed by transformation of chemically competent Escherichia coli DH5α cells [ ]. Article Title: Molecular cloning, the characterization of metallothionein and catalase, and the evaluation of testicular toxicity of Cd in the Chinese fire-bellied newt (Cynops orientalis). Article Snippet: All products that were produced by the PCRs were isolated via electrophoresis, purified using Article Title: Genome editing in rice and wheat using the CRISPR/Cas system. Article Snippet: nature protocols | VOL.9 NO.10 | 2014 | 2395 IntroDuctIon In recent years, genome editing technologies, ZFNs1–3 and TALENs4–8 have been used to mutate specific loci in various plants9–16.. Both ZFNs and TALENs are fusion proteins that consist of a DNA-binding domain fused to the FokI endonuclease domain.. The endonuclease domain induces DNA double-strand breaks (DSBs) at targeted sites in the genome, which are determined by the binding specificity of the DNA-binding domain. Article Title: Wheat plants resistant to powdery mildew Article Snippet: The PCR products amplified with TaMLO-specific primers (Table 3) from individual mutant plants were cloned into Gel Extraction:Article Title: miR-29c-3p regulates DNMT3B and LATS1 methylation to inhibit tumor progression in hepatocellular carcinoma Article Snippet: The PCR products were electrophoresed, purified using Spin‐X tubes, and then cloned into the pUC-T vector (both from CWbiotech, Beijing, China). Article Title: Modified plants Article Snippet: The PCR products amplified with TaMLO-specific primers (Table 3) from individual mutant plants were cloned into Article Title: Methylation-associated inactivation of JPH3 and its effect on prognosis and cell biological function in HCC Article Snippet: The PCR products were electrophoresed, purified using Spin-X tubes, and then cloned into the Article Title: Advances in Cereal Crop Genomics for Resilience under Climate Change Article Snippet: , AMT , MLO , NHEJ , TaU6 , Ubi , Article Title: Complete nucleotide sequence of strawberry vein banding virus Chinese isolate and infectivity of its full-length DNA clone Article Snippet: The PCR products with a 3′-A addition were inserted into pUC-T Vector (CWBIO, Beijing, China) using TA-cloning strategy followed by transformation of chemically competent Escherichia coli DH5α cells [ ]. Article Title: Molecular cloning, the characterization of metallothionein and catalase, and the evaluation of testicular toxicity of Cd in the Chinese fire-bellied newt (Cynops orientalis). Article Snippet: All products that were produced by the PCRs were isolated via electrophoresis, purified using Article Title: Genome editing in rice and wheat using the CRISPR/Cas system. Article Snippet: nature protocols | VOL.9 NO.10 | 2014 | 2395 IntroDuctIon In recent years, genome editing technologies, ZFNs1–3 and TALENs4–8 have been used to mutate specific loci in various plants9–16.. Both ZFNs and TALENs are fusion proteins that consist of a DNA-binding domain fused to the FokI endonuclease domain.. The endonuclease domain induces DNA double-strand breaks (DSBs) at targeted sites in the genome, which are determined by the binding specificity of the DNA-binding domain. Article Title: Wheat plants resistant to powdery mildew Article Snippet: The PCR products amplified with TaMLO-specific primers (Table 3) from individual mutant plants were cloned into Transformation Assay:Article Title: miR-29c-3p regulates DNMT3B and LATS1 methylation to inhibit tumor progression in hepatocellular carcinoma Article Snippet: The PCR products were electrophoresed, purified using Spin‐X tubes, and then cloned into the pUC-T vector (both from CWbiotech, Beijing, China). Article Title: Modified plants Article Snippet: The PCR products amplified with TaMLO-specific primers (Table 3) from individual mutant plants were cloned into Article Title: Methylation-associated inactivation of JPH3 and its effect on prognosis and cell biological function in HCC Article Snippet: The PCR products were electrophoresed, purified using Spin-X tubes, and then cloned into the Article Title: Advances in Cereal Crop Genomics for Resilience under Climate Change Article Snippet: , AMT , MLO , NHEJ , TaU6 , Ubi , Article Title: Complete nucleotide sequence of strawberry vein banding virus Chinese isolate and infectivity of its full-length DNA clone Article Snippet: The PCR products with a 3′-A addition were inserted into pUC-T Vector (CWBIO, Beijing, China) using TA-cloning strategy followed by transformation of chemically competent Escherichia coli DH5α cells [ ]. Article Title: Molecular cloning, the characterization of metallothionein and catalase, and the evaluation of testicular toxicity of Cd in the Chinese fire-bellied newt (Cynops orientalis). Article Snippet: All products that were produced by the PCRs were isolated via electrophoresis, purified using Article Title: Genome editing in rice and wheat using the CRISPR/Cas system. Article Snippet: nature protocols | VOL.9 NO.10 | 2014 | 2395 IntroDuctIon In recent years, genome editing technologies, ZFNs1–3 and TALENs4–8 have been used to mutate specific loci in various plants9–16.. Both ZFNs and TALENs are fusion proteins that consist of a DNA-binding domain fused to the FokI endonuclease domain.. The endonuclease domain induces DNA double-strand breaks (DSBs) at targeted sites in the genome, which are determined by the binding specificity of the DNA-binding domain. Article Title: Wheat plants resistant to powdery mildew Article Snippet: The PCR products amplified with TaMLO-specific primers (Table 3) from individual mutant plants were cloned into T7EI Assay:Article Title: miR-29c-3p regulates DNMT3B and LATS1 methylation to inhibit tumor progression in hepatocellular carcinoma Article Snippet: The PCR products were electrophoresed, purified using Spin‐X tubes, and then cloned into the pUC-T vector (both from CWbiotech, Beijing, China). Article Title: Modified plants Article Snippet: The PCR products amplified with TaMLO-specific primers (Table 3) from individual mutant plants were cloned into Article Title: Methylation-associated inactivation of JPH3 and its effect on prognosis and cell biological function in HCC Article Snippet: The PCR products were electrophoresed, purified using Spin-X tubes, and then cloned into the Article Title: Advances in Cereal Crop Genomics for Resilience under Climate Change Article Snippet: , AMT , MLO , NHEJ , TaU6 , Ubi , Article Title: Complete nucleotide sequence of strawberry vein banding virus Chinese isolate and infectivity of its full-length DNA clone Article Snippet: The PCR products with a 3′-A addition were inserted into pUC-T Vector (CWBIO, Beijing, China) using TA-cloning strategy followed by transformation of chemically competent Escherichia coli DH5α cells [ ]. Article Title: Molecular cloning, the characterization of metallothionein and catalase, and the evaluation of testicular toxicity of Cd in the Chinese fire-bellied newt (Cynops orientalis). Article Snippet: All products that were produced by the PCRs were isolated via electrophoresis, purified using Article Title: Genome editing in rice and wheat using the CRISPR/Cas system. Article Snippet: nature protocols | VOL.9 NO.10 | 2014 | 2395 IntroDuctIon In recent years, genome editing technologies, ZFNs1–3 and TALENs4–8 have been used to mutate specific loci in various plants9–16.. Both ZFNs and TALENs are fusion proteins that consist of a DNA-binding domain fused to the FokI endonuclease domain.. The endonuclease domain induces DNA double-strand breaks (DSBs) at targeted sites in the genome, which are determined by the binding specificity of the DNA-binding domain. Article Title: Wheat plants resistant to powdery mildew Article Snippet: The PCR products amplified with TaMLO-specific primers (Table 3) from individual mutant plants were cloned into |